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How to make a blood smear

Written by Ines Dec 26, 2020 ยท 10 min read
How to make a blood smear

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How To Make A Blood Smear. Clean the area to be punctured with 70 alcohol. How to perform a blood film in 3 easy stepsLearn more and visit httpswwwidexxcouk. Do not touch the surface of the slide where the blood smear will be made. Can occur with anemic patients Increase the angle of the slide you are using to smear with.

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Do not touch the surface of the slide where the blood smear will be made. To make a short smear hold the spreader at a steeper angle and to make a longer smear hold it closer to the drop. Clean slides with 70 to 90 alcohol and allow to dry. They then tie a band above. Common cause of a poor blood smear. Preparation of Blood Film.

Select the finger to puncture usually the middle or ring finger.

The scratches allow for improved adherence of the blood film to the slide without affecting the smear morphology. Aim for a blood droplet size of 4mm diameter. The feathered edge the monolayer and the body and base of the smear. Optimise spreading speed for length and a good feathered edge. Take another clean slide and holding at an angle of about 45 deg touch the blood with one end of the slide so the blood runs along the edge of the slide by capillary action. 3 Place the end of the spreader slide on the sample slide so that the short sided edge of the spreader is below the drop of blood.

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The main part of the blood film. They then tie a band above. Place a small drop of blood or one side about 1-2 cm from one end. The main part of the blood film. Optimise spreading speed for length and a good feathered edge.

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Without delay place a spreader at an angle of 45 from the slide and move it back to make contact with the drop. Blood Smear Technique 1 On one slide the sample slide place a small drop of blood 2 Use the second slide as a spreader slide. The portion of blood film near the drop of blood. Take one drop of venous or capillary blood. The process is similar to making a normal thick film but instead of using a stick to spread the blood the edge of a glass microscope slide is used while applying firm pressure to create small scratches in the underlying slide.

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Always start with room temperature well-mixed clot free EDTA anticoagulated blood heparin is used for some exotic species. Select the finger to puncture usually the middle or ring finger. In this video I quickly go over how to make a peripheral blood smear. To make a short smear hold the spreader at a steeper angle and to make a longer smear hold it closer to the drop. Spreader slide pushed across the slide in a jerky.

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Do not touch the surface of the slide where the blood smear will be made. Aim for a blood droplet size of 4mm diameter. Preparation of Blood Film. Collect blood in an EDTA tube and make the smears when back at the clinic. A well made blood smear consists of several areas.

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Can occur with anemic patients Increase the angle of the slide you are using to smear with. Parts of Blood Smear A peripheral blood film consists of 3 parts. Take another clean slide and holding at an angle of about 45 deg touch the blood with one end of the slide so the blood runs along the edge of the slide by capillary action. The process is similar to making a normal thick film but instead of using a stick to spread the blood the edge of a glass microscope slide is used while applying firm pressure to create small scratches in the underlying slide. A phlebotomist a person specifically trained to draw blood first cleans and sterilizes the injection site with an antiseptic.

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How to Make a Peripheral Blood Smear. They then tie a band above. The tapering end of the blood film. The process is similar to making a normal thick film but instead of using a stick to spread the blood the edge of a glass microscope slide is used while applying firm pressure to create small scratches in the underlying slide. Push the edge of the pusher slide into the blood drop so that it spreads out completely along the pusher slide.

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Without delay place a spreader at an angle of 45 from the slide and move it back to make contact with the drop. Place a small drop of blood or one side about 1-2 cm from one end. The main part of the blood film. To make a short smear hold the spreader at a steeper angle and to make a longer smear hold it closer to the drop. Prick the ring finger of left hand with the help of prickling needle under all aseptic conditions discard the first two drOps of blood from skin puncture site.

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A phlebotomist a person specifically trained to draw blood first cleans and sterilizes the injection site with an antiseptic. Take another clean slide and holding at an angle of about 45 deg touch the blood with one end of the slide so the blood runs along the edge of the slide by capillary action. The process is similar to making a normal thick film but instead of using a stick to spread the blood the edge of a glass microscope slide is used while applying firm pressure to create small scratches in the underlying slide. Place either the long or the short edge of the pusher slide just in front of the blood drop holding it at an approximately 30-degree angle from the patient slide. Making a quality blood smear Although there are several techniques described for making blood smears most people use the wedge or push technique.

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If the blood has been refrigerated allow it to return. Place a small drop of blood or one side about 1-2 cm from one end. Specimen of blood can also be taken by venepuncture. Proper preparation of the blood smear is critical for correct interpretation and obtaining accurate results. Place a drop of blood on the patient slide near the frosted edge.

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Place a drop of blood on the patient slide near the frosted edge. Place a drop of blood on the patient slide near the frosted edge. How to Make a Peripheral Blood Smear. Clean the area to be punctured with 70 alcohol. Place a small drop of blood or one side about 1-2 cm from one end.

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Place a small drop of blood or one side about 1-2 cm from one end. A quick instructional video demonstrating. Put a small size of blood drop on each glass slide about half centimeter from its narrow edge on the right side. How to perform a blood film in 3 easy stepsLearn more and visit httpswwwidexxcouk. The feathered edge the monolayer and the body and base of the smear.

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A well made blood smear consists of several areas. Now push the spreader across the slide. Making a quality blood smear Although there are several techniques described for making blood smears most people use the wedge or push technique. Blood Smear Technique 1 On one slide the sample slide place a small drop of blood 2 Use the second slide as a spreader slide. Clean slides with 70 to 90 alcohol and allow to dry.

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Push carefully along the length of the first slide to produce a thin smear of blood. Blood Smear Technique 1 On one slide the sample slide place a small drop of blood 2 Use the second slide as a spreader slide. Take another clean slide and holding at an angle of about 45 deg touch the blood with one end of the slide so the blood runs along the edge of the slide by capillary action. Do not touch the surface of the slide where the blood smear will be made. How to perform a blood film in 3 easy stepsLearn more and visit httpswwwidexxcouk.

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Common cause of a poor blood smear. Optimise spreading speed for length and a good feathered edge. Drop of blood too large or too small 2. The feathered edge the monolayer and the body and base of the smear. Making a quality blood smear Although there are several techniques described for making blood smears most people use the wedge or push technique.

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The portion of blood film near the drop of blood. Specimen of blood can also be taken by venepuncture. The drop and it spreads by capillary action along its edge. Otherwise your smear may run right off of your horizontal slide and you will not have a nice feathered edge and your smear will be so thin you will have trouble finding enough cells. To make a short smear hold the spreader at a steeper angle and to make a longer smear hold it closer to the drop.

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Proper preparation of the blood smear is critical for correct interpretation and obtaining accurate results. Place a drop of blood on the patient slide near the frosted edge. They then tie a band above. Take another clean slide and holding at an angle of about 45 deg touch the blood with one end of the slide so the blood runs along the edge of the slide by capillary action. Blood can also be taken from an EDTA sample.

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Do not touch the surface of the slide where the blood smear will be made. The tapering end of the blood film. All parts of the smear should be examined however the monolayer is the area where the cells are examined in close detail and differential cell. Place a small drop of blood or one side about 1-2 cm from one end. The drop and it spreads by capillary action along its edge.

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A quick instructional video demonstrating. The feathered edge the monolayer and the body and base of the smear. To make a short smear hold the spreader at a steeper angle and to make a longer smear hold it closer to the drop. Specimen of blood can also be taken by venepuncture. Preparation of Blood Film.

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